Study on Extraction Method of Total RNA from Peony Buds
Hu Xiaorong
Ma Yan
Zang Dekui
Song Shuxiang
Guo Xianfeng
Abstract：The total RNA was extracted from dormancy and dormancy -released peony buds by modified CTAB method , Trizol method and EASYspin Plus RNA rapid isolation kit respectively .The quality of the ex-tracted RNA was tested by protein nucleic acid analyzer , agarose gel electrophoresis and RT -PCR.The re-sults showed that the total RNA could not be extracted by Trizol method , and that extracted by EASYspin Plus RNA rapid isolation kit was too lower to meet the requirement of further experiment .The total RNA extracted by modified CTAB method was more complete with clearer bands and better quality , and the brightness of 28 S rRNA was about 2 times of that of 18S rRNA with no degradation .Using the total RNA extracted by modified CTAB method as template to amplify the internal reference gene Actin, the obtained bands were clear and with the same size to the target fragment , which further proved that the total RNA extracted by modified CTAB method could meet the requirement of the later experiment .
Keywords：Peony budTotal RNAExtractRT-PCR
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 16-20 )