Survey on Root Endophytic Fungi Resources of Vaccinium uliginosum Linn.
Liu Fenghong
Cheng Xianhao
Gu Liang
Feng Peiyong
Shao Xiaohuan
Zhao Qing
Abstract：The mycorrhizal morphology and distribution of endophytic fungi in wild Vaccinium uliginosum Linn.in Changbai Mountain were studied through tabletting method.The endophytic fungi were isolated through root segment culturing method and grinding method, and were identified and analyzed based on fungal morphology and molecular biology technique.The results showed that the mycorrhizal infection rate was as high as 75%.According to the morphology of hyphae, the wild Vaccinium uliginosum Linn.contained at least three kinds of endophytic fungi, among which, thick hyphae formed dense hypha body, loose thick hyphae ran through the cell and thin hyphae formed loose hypha body.A total of 175 strains of endophytic fungi were iso-lated.Through morphology and molecular identification, 150 strains were divided into 8 species in 5 genera, among which, Phialocephala spp.was the dominant genera with the frequency of 68.57%.The amount of en-dophytic fungi isolated by root segment grinding method was larger than that of root segment culturing method, and that isolated from thin mycorrhiza was larger than that from thick mycorrhiza.The isolation of plentiful en-dophytic fungi laid foundations for further screening, development and utilization of bio-control strains.
Keywords：Vaccinium uliginosum Linn.MycorrhizaEndophytic fungiMorphological characteris-ticsMolecular identification
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 41-46 )