Optimization of SRAP -PCR Amplification System for Pyrus Wild Germplasms
Liang Tingting
Ma Yan
Zang Dekui
Abstract：Taking Pyrus wild germplasms as materials, four levels of five factors influencing SRAP am-plification system were optimized using the orthogonal design-direct analysis and Duncan’s new multiple range tests.The five factors were as the concentrations of Mg2+, dNTPs, primers, Taq DNA polymerase and tem-plate DNA.The results showed that the optimized SRAP reaction system was 25 μL and contained 10 ×PCR buffer 2.5 μL, 2.0 mmol/L Mg2+, 200 μmol/L dNTPs, 0.4 μmol/L primers, 1.5 U Taq DNA polymerase and 60 ng template DNA.
Keywords：PyrusSRAPOrthogonal design-direct analysisDuncan’s new multiple range tests
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 7-10 )