Cloning and Sequence Analysis of CBF Gene from Cherry
Zhang Jianpeng
Chen Xin
Xu Li
Jia Jianyun
Zhu Dongzi
Zong Xiaojuan
Wei Hairong
Liu Qingzhong
Abstract：CBF ( C-repeat binding transcription factor ) is a transcription factor in cold acclimation re-sponse .CBF proteins combine with a lot of low temperature related promoters to express these genes , thus the plant tolerance to cold could be increased .In this study , the specific primers designed according to the con-served sequence of CBF gene from other plants were used to amplify the cDNA of Gisela 6, and then the total length sequence of CBF was obtained successfully .The sequence analysis results showed that its open reading frame ( ORF) was 723 bp encoding 240 amino acids , and it possessed two short peptide sequences and the specific AP2 domain protein .The amino acid sequence homology analysis showed that the cloned CBF shared higher homology with that from Rosaceae but lower with that from other plants .
Keywords：CherryCBF geneCloneSequence analysis
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )