Study on Key Starch Degrading Enzyme Activity and Related Gene Expression Level during Early Germination Stage of Wheat Seed
Liu Mei
Zhang Feng
Yang Cuicui
Yang Wensi
Sun Qingquan
Abstract：Shannong17 was used to investigate the activities of key enzymes and expression levels of re-lated genes of starch degradation during early germination stage of wheat seed .The contents of starch and solu-ble sugar and the activities of starch degradation enzymes were determined in germinating wheat seeds at differ -ent temperatures;their related gene expression levels were detected by real -time fluorescence quantitative PCR.The results showed that the starch content decreased at different rates with the germination process at different temperatures;the soluble sugar content decreased at first and then increased;the activities ofα-am-ylase and starch phosphorylase and the respiration rate kept increasing with the process of germination .Obvi-ous changes of these indicators were found during the period of visible radicle protrusion through the seed cov -ering layers.The activity of β-amylase demonstrated a double -peak curve and kept highly active during germination.The relative gene expression level of starch degradation enzyme (except β-amylase) increased with the process of germination , and was significantly correlated with the activities of responded enzymes .In conclusion , the relative gene expression levels of the key starch degradation enzymes were affected strongly by temperature at the early stage of wheat seed germination .The activities of α-amylase and starch phosphoryl-ase which played important roles during germination could be detected at the initial period of germination and were regulated greatly by its encoding genes.
Keywords：Starch metabolismAmylaseStarch phosphorylaseReal-time fluorescence quantitative PCRGene expression
Publication Date：2014-09-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 39-45 )