Cloning and Transient Expression Analysis of WRKY-R1 Gene from Tobacco (Nicotiana tabacum L.)
Hu Man
Song Dandan
Yang Jinmiao
Liu Weiqun
Abstract：The intact ORF of NtWRKY-R1 gene was isolated by RT-PCR combined with in silico clo-ning from tobacco root tip tissues .Sequence analysis showed that NtWRKY -R1 had conserved WRKYGOK sequence and a C2H2 -type zinc finger motif , and belonged to group Ⅱ WRKY transcription factor family . Bioinformatics methods were used to predict and analyze the physical and chemical characters , evolutionary re-lationships and phosphorylation sites of NtWRKY -R1 protein.The results showed NtWRKY -R1 had a clos-er affinity with Solanaceae in homology and evolutionary relationships .Phosphorylation site analysis showed that NtWRKY-R1 might regulate corresponding metabolic activity through phosphorylation modification .By constructing eukaryotic expression vector and establishing transient expression analysis system , the over-ex-pression of NtWRKY-R1 gene resulted in down-regulated expression of nicotine biosynthesis key gene PMT1 and JA signaling pathway marker gene PDF1.2.It was thought that NtWRKY-R1 gene affected JA signaling pathway and then regulated nicotine biosynthesis .
Keywords：Nicotiana tabacum L.NtWRKY-R1Gene cloningBioinformatics analysisTransient expression analysisSignaling pathway
Publication Date：2014-09-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 12-16,20 )