Prokaryotic Expression, and RNAi Vector’s Construction and Transfor-mation of PnLOX2 Gene from Peanut with Resistance to Aspergillus flavus
Zhang Tingting
Ma Dengchao
Gong Qingxuan
Li Chunjuan
Yan Caixia
Zhao Xiaobo
Shan Shihua
Abstract：PnLOX2,a differential expression gene was previously isolated from two peanut cultivars re-sistant or susceptible to Aspergillus flavus by gene chip technology .It was expressed in E.coli BL21 and the fu-sion protein of 121 .5 kD was obtained .At the same time, its inverted repeats of the 3′end were constructed and transformed into peanut via Agrobacterium tumefaciens mediated transformation method .The primary trans-genic peanut plants were obtained .This study laid foundations for reverse identification of the function of Pn-LOX2 gene in the process of peanut resisting Aspergillus flavus infection.
Keywords：PeanutResistance to Aspergillus flavusProkaryotic expressionInverted repeats
Publication Date：2014-09-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )