Fine Mapping of Fertility Restorer Gene Rf3 of S-Type Cytoplasmic Male Sterility and Candidate Gene Prediction in Maize
Li Peng
Xiao Senlin
Wang Shuxia
Liu Juan
Zhao Xianrong
Chen Huabang
Abstract：Cytoplasmic male sterility ( CMS) is widespread in higher plants .CMS-S, one of the major CMS in maize , can be restored by Rf3 gene which has not been cloned yet .Based on the gametophytic nature of maize CMS-S, a novel homogeneous population constructing method was put forward for mapping .All the individuals obtained through this method carried the Rf3 gene making the phenotype identification unnecessa-ry , and enough recombinants could be obtained in the same generation .Using this method , the DNA extracted from seeds could be used directly , so the efficiency of Rf3 mapping could be improved greatly .With this hom-ogeneous population , Rf3 gene could be fine mapped in the locus between molecular marker A 165 and CG2 . The physical distance between the two SSR markers was about 1.4 Mb according to the genome sequence of maize inbred line B73.
Keywords：MaizeCytoplasmic male sterility ( CMS)S typeRestorer gene
Publication Date：2014-08-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )