Optimization of Rapid DNA Extraction Methods from Peanut Leaves
Wang Lei
Zhao Xintao
Xu Mengqi
Su Feng
Ren Yan
Li Shuangling
Shi Yanmao
Yuan Mei
Wang Chuantang
Abstract:With the young leaves of peanut as experiment material , four different DNA extraction meth-ods, the high-throughput DNA extraction method, the simple SDS method, TPS method and TENP method, were compared and optimized .The results showed that , compared with the other improved methods , the im-proved TPS method had the advantages of grinding samples with no liquid nitrogen , faster extraction rate , fe-wer kinds of reagent , lower cost, rapider DNA solution rate and better DNA quality .The DNA extracted through improved TPS method could be used for SSR analysis of peanut .
Keywords:Peanut ( Arachis hypogaea L.)Genomic DNAExtraction methodYoung leaves
Publication Date:2014-07-02
Online Publishing Date:2026-05-22(First online date of this platform, not the publication date of the document)
Pages:4( 11-14 )
