Construction of Polygenic Plant Expression Vector for Synthesis of DGLA and ETA
Sun Mizhen
Li Xinzheng
Zhou Jiajia
Sun Xuezhen
Song Xianliang
Li Mingchun
Abstract：The Δ8 desaturase (Δ8Des) andΔ9 elongase (Δ9Elo) are the key enzymes for the synthesis of dihomo-γ-linolenic acid (DGLA) and eicosatetraenoic acid (ETA).In the research, a new assistant ex-pression vector, PAUX-2, was constructed through replacing the 35S promoter with a soybean seed -specific promoter BCSP952.Then a plant expression vector , pCambia2300-Δ8Δ9, simultaneously containing Δ8Des gene from Euglena gracilis andΔ9Elo gene from Isochrysis galbana, was constructed , which provided essential tool to study the expression of these exogenous genes in plants .
Keywords：Very -long -chain polyunsaturated fatty acids ( VLCPUFAs )Δ8 desaturase geneΔ9 elongase geneSeed-specific promoterExpression vector
Publication Date：2014-07-02
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )