Construction of Genetic Linkage Map Based on SSR Marker in Maize F2 Population Derived from QR-001 and QS-001 Crossing
Gong Yuan
Lan Jinhao
Abstract:The inbred line QR-001 with high resistance to maize rough dwarf virus ( MRDV) as female parent , and the highly susceptible inbred line QS -001 as male parent , were used to produce a F2 segregation mapping population including 281 individuals .1 028 pairs of SSR primers , which distributed evenly though -out the genome in maize ( Zea mays L.) , were used for screening polymorphisms between the parental lines . And 110 polymorphic SSR markers were identified .The linkage group covering 10 chromosomes were construc-ted using the software QTL IciMapping Version 3.3.The total length of linkage group was 1 343.2 cM, and the even distance of each marker interval was 10 .3 cM.Only 1 molecular marker ( bnlg161 ) was not linked with the population .There were 26 .6%of all the SSR markers occurring segregation distortion .The linkage group was helpful for the MRDV QTL mapping , molecular marker-assisted selection and molecular design of resist-ance breeding .
Keywords:MaizeGermplasm resistant to maize rough dwarf virusF2 populationSSR molecular markerGenetic linkage map
Publication Date:2014-06-02
Online Publishing Date:2026-05-22(First online date of this platform, not the publication date of the document)
Pages:5( 11-15 )
