Cloning and Sequence Analysis of Seed-Specific Promoter in Cotton
Zhou Jiajia
Sun Mizhen
Zhang Suqing
Yi Haifa
Xing Huixian
Wang Liyuan
Song Xianliang
Sun Xuezhen
Abstract：Promoter is a crucial cis-regulatory element of gene expression .In genetic engineering , seed-specific promoter plays an important role in regulating the specific expression of exogenous genes in seeds , im-proving the expression efficiency and enhancing the transgenic effect .In this paper , a seed-specific promoter sequence of gene D34 was cloned from Gossypium barbadense by touch down PCR amplification with the prim-ers designed based on the D34 gene of cotton LEA protein .Sequence analysis showed that the cloned fragment was 1 384 bp in length and similar to published sequence with the similarity as 94 .87%.The cloned segment not only contained promoter basic components such as TATA box , CAAT box, but also contained seed -spe-cific promoter components such as E -box , G-box and AACA motif .The biological function of these cis -el-ements was also analyzed .
Keywords：CottonSeed-specific promoterSequence analysis
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 6-10 )