Cloning and Sequence Analysis of Clp Protease Gene (TaClpP) from Wheat (Triticum aestivum L.)
Zhang Bin
Yang Lianqun
Bian Fei
Li Nana
Fan Zhongxue
Gong Yongchao
Pu Yanyan
Ding Hanfeng
Peng Zhenying
Abstract：Clp protease ( ClpP) widely exists in various prokaryotes and eukaryotes , and plays important roles in the regulation of protein metabolism .In this study , a TaClpP gene ( GenBank No .KJ541961 ) was cloned from wheat ( Triticum aestivum L.) leaves by homology cloning strategy and RACE method .The cDNA sequence analysis showed that TaClpP had a single open reading frame of 897 bp; the predicted protein had 298 amino acids and the predicted molecular mass was 32 .6 kD;the isoelectric point was 9 .79 .This predic-ted protein contained a conserved S14-ClpP-2 domain motif, and had no signal peptide in its N -terminus. The supposed subcellular location of TaClpP was in chloroplast .Compared with other plants ’ ClpP members, TaClpP had high homology with its counterpart of Aegilops tauschii and Triticum urartu.In addition, the TaClpP genome sequence was obtained by PCR amplification , which contained 9 exons and 8 introns, and the length was 3 256 bp.
Keywords：Triticum aestivum L.Clp protease geneGene cloningSequence analysis
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )