Research on Establishment of in vitro Regeneration System of Tomato and Control of Browning Phenomenon
Wang Lei
Su Feng
Abstract：A complete set of in vitro propagation system of tomato was established and the browning phe-nomenon in this process was studied.The results showed that the optimum explant for callus inducing was cot-yledon,and the optimum plant growth regulator was 0.6 mg/L IAA+2 mg/L 6-BA.The 6-day cotyledon had the best callus induction effect.The optimum plant growth regulator concentration for budding was 0.3 mg/L IAA+2 mg/L 6 -BA.Adding 200,600 mg/L activated carbon or 10 mg/L VC into medium,the browning phenomenon could be controlled effectively in the formation of calluses and adventitious buds.The optimum rooting method was indirect rooting method,and its optimum NAA concentration was 50 mg/L.Un-der these optimum conditions,a whole regenerated plantlet could be obtained in about 50 days.
Keywords：TomatoTissue cultureBrowning
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 9-12,16 )