Rapid Detection of Resistant Gene Ty-1 in Tomato by PCR
Jiang Zhen
Jin Ping
Cheng Fei
Gao Jianwei
Abstract：The resistant gene Ty-1 of homozygous resistant lines“JZ-108”(Ty-1/Ty-1 ),homozy-gous susceptible lines “1712”(ty-1/ty-1)and their F1 generation was amplified by PCR method using 10 pairs of marker primers published at home and abroad.The results showed that SSR47 was the specific primer which had a 750 bp amplification fragment in homozygous resistant lines,a 640 bp fragment in susceptible lines and both 750 bp and 640 bp fragments in heterozygous resistant lines.The detection results were com-pletely consistent with those of field identification.So this co -dominant marker,tightly linked to Ty-1 gene,could distinguish homozygous and heterozygous resistant lines and susceptible lines.Forty-eight indi-viduals from F2 generation of“JZ-108 ×1712”were detected.There were 8 homozygous resistant lines,19 homozygous susceptible lines and 2 1 heterozygous resistant lines.Both homozygous and heterozygous resistant lines displayed resistance to tomato yellow leaf curl disease in the field.The replicated stable results proved that SSR47 could be used for rapid identification of Ty-1 resistant gene in tomato.
Keywords：Tomato yellow leaf curl diseaseTy-1PCR
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 5-8,45 )