Involvement of Proline Metabolic Pathway in Regulating Drought Tolerance in Rice phyB Mutant
Zhou Jinjun
Liu Peng
Yin Jingjing
Xie Xianzhi
Abstract：The expression levels of key genes involved in proline metabolic pathway were compared in rice wild type (WT)and phyB mutant.The results showed that dehydration treatment induced the expression of proline biosynthetic gene OsP5CS1 and OsOAT,but suppressed the expression of proline catabolic gene OsP5CDH.And the expression level of OsP5CS1 gene in phyB mutant was obviously higher than that in WT, which suggesting that phyB negatively regulated its expression.To explore the relation between high OsP5CS1 transcript level and stronger drought tolerance in phyB mutant,the OsP5CS1 transgenic tobacco was obtained. Water loss rate assays of detached leaves revealed that transgenic tobacco showed a slower water loss rate com-pared to non-transgenic tobacco.In addition,the leaf disk differentiation rate under high salinity of transgen-ic tobacco was higher than that of non -transgenic tobacco.These findings suggested that the regulation of phyB on proline metabolic pathway was one of the important factors contributing to stronger drought tolerance in phyB mutant.
Keywords：RicephyB mutantProlineDroughtSalt stress
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )