Establishment and Application of RT-PCR for Detection of Bovine Enterovirus
Hou Peili
Cheng Hongbing
Liu Xiao
Wang Hongmei
He Hongbin
Abstract：A reverse transcription PCR (RT-PCR) method for detection of bovine enterovirus (BEV) was established using a pair of specific primers designed based on the 3 D gene of BEV in GenBank .This method could specifically amplify a 732-bp fragment from BEV strain , but not from bovine viral diarrhea vi-rus (BVDV), infectious bovine rhinotracheitis virus (IBRV), bovine coronavirus (BCoV) and bovine rota-virus virus (BRV).The detection limit of this method was 10 -1 TCID50 of BEV.In a detection of 84 samples suspected with bovine disease , 21 samples were positive with the positive rate as 25%.
Keywords：Bovine enterovirus3D geneRT-PCRDetection
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )