Construction of Wheat Transgenetic Technology System using Isolated Anthers as Particle Bombardment Receptor
Gao Jie
Wang Jiao
Zhang Shujuan
Guo Dong
Li Yulian
Song Guoqi
Chen Xueyan
Li Genying
Abstract：To obtain homozygous transgenic wheat within one generation , the parameters involved in bombardment and chromosome doubling were studied in details .The results indicated that the best parameter combination for anther bombardment were 0.6-μm particle size, 100 μg per shot, 6-cm bombardment dis-tance and 1 300-psi bombardment pressure .The highest doubling efficiency ( 100%) was obtained when haploid plants were treated with 0.2%colchicine solution containing 5%DMSO for 4 hours, and that contai-ning 1%DMSO had the lowest doubling efficiency (62.2%).Specific PCR amplification showed that all T 0 positive plants had no segregation in T 1 generation, which indicated that the homozygous transgenic lines were obtained within one generation .This study established a successful transgenic technology system using isolated wheat anthers as bombardment recipient , which provided an efficient approach for wheat genetic improvement .
Keywords：WheatTransgenosisReceptorAntherChromosome doubling
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-5,18 )