Clinical Efficacy of Zizhu Ointment(紫朱软膏)in Non-Ischemic Diabetic Foot Ulcer Treatment and Its Effect on Expression of Ferroptosis-Related Biomarkers
HUANG Hechen
ZHOU Shaorong
LIU Guobin
HU Xiaoming
PAN Qiong
XU Zhaodong
LI Zhuo
WANG Yuancheng
XU Liyan
Abstract：Objective:To evaluate the clinical efficacy and safety of Zizhu Ointment(紫朱软膏)in non-ischemic diabetic foot ulcer(DFU)treatment and its effect on the expression of ferroptosis-related biomarkers in patients with wound.Methods:According to the random number table method,all patients were allocated into the observation group and control group,with 53 cases in each group.On the premise of routine treatment combined with syndrome differentiation and internal treatment by Chinese medicine,Zizhu Ointment gauze dressings were applied in the observation group,while rb-bFGF combined with petroleum gauze dressings were applied in the control group.Dressings were changed once a day for 8 weeks in both group.The clinical efficacy,wound area,DMIST scale score,GSH and MDA content in wound tissue of the two groups were observed before and after treatment.Immunohistochemical changes of GPX4,SLC7A11 and TFRC in wound tissue were also compared between before and after treatment in the observation group.Adverse events were recorded during the treatment,and safety evaluation was conducted after the treatment.Results:On the clinical efficacy,wound area,DMIST scale score,GSH and MDA content in wound tissue,the observation group was superior to the control group(P＜0.05).Positive expressions of GPX4 and SLC7A11 in wound tissue were increased in the observation group while TFRC expression was decreased(P＜0.05).There was no adverse event in both groups.Conclusions:Zizhu Ointment is applicable for non-ischemic DFU in all courses.It is with good clinical efficacy and high safety,for it can reduce the wound area,improve DMIST scale score of the wound,enhance the antioxidant capacity of wound tissue,reduce the level of lipid peroxidation,and play a certain role in regulating the biomarkers related to ferroptosis in the process of wound healing.
Keywords：diabetic foot ulcerZizhu Ointmentferroptosisexternal treatment of traditional Chinese medicineantioxidantlipid peroxidation
Publication Date：2025-10-05
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：10( 1127-1136 )