Effect of Bushen Huoxue Tongfu Formula on Protein Expression in SIRT1 Signaling Pathway in Rat Model of Chronic Cerebral Blood Deficiency
YANG Haihao
LIU Mingjun
HAN Tao
HUANG Hailiang
LIU Xijian
LYU Zheng
Abstract：Objective:To study the protective effect of Bushen Huoxue Tongfu formula(BHTF)on brain tissue of rats with chronic cerebral circulatory insufficiency(CCCI). Methods:Fifty healthy male Wistar rats were randomly divided into five groups:sham operation group,model group,nimodipine group,BHTF of low dose group,BHTF of high dose group. The CCCI rat model was established using 2-VO permanent ligation. After 8 weeks of intragastric administration,the rats were sacrificed through decapitation,and the hippocampus was harvested. The protein was detected by Western blot. Results:Compared with the sham group,the expression of PGC-1α and FOXO3α in the frontal lobe of the model group and other treatment groups were decreased significantly(P<0.05). Compared with the model group,the expression of PGC-1α protein in the hippocampus of the BHTF of low dose group was significantly higher than that in the model group(P<0.05). Compared with the nimodipine group,FOXO3α,PGC-1α protein expressions were significantly increased(P<0.05). Con-clusion:Cerebral ischemia in CCCI rats can inhibit the expressions of FOXO3α and PGC-1α proteins. BHTF of low dose can improve the expressions of FOXO3α and PGC-1α proteins. Increase the blood supply of the brain,thereby protecting the brain tissue of CCCI rats.
Keywords：chronic cerebral circulatory insufficiencyBushen Huoxue Tongfu formularat
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 1063-1066 )