Effect of Governor-Moxibustion on Learning and Memoryand HE Staining in Hippocampal CA1 and CA3 Area of Mouse with Alzheimer's Disease

LIU Yuanxiang; YANG Jiguo

Abstract：Objective:To investigate the effect of governor-moxibustionon learning and memory ability and HE staining in hippocampal CA1 and CA3 of Alzheimer's disease model mice. Methods:Forty SAMP8 mice were randomly divided into normal group,model group,governor-moxibustion group,Chinese herbal medicinegroup and governor moxibustion plus Chinese herbal medicine group,8 mice in each group. In the governor-moxi-bustion group the mice were given governor-moxibustion and in the Chinese herbal medicine group the mice were given Liuwei Dihuang pill through gavage. At the end of the experiment,Morris water maze test was used to conduct behavioral testing. HE staining was used to detect the histological changes in CA1 and CA3 areas. Results:Compared with the model group,the escape latency of the Chinese herbal medicine group, governor-moxibustion group and governor moxibustion plus Chinese herbal medicine group were all decreased (P<0.05 or P<0.01),and that of governor-moxibustion group and the governor-moxibustion plus Chinese herbal medicine group were the shortest,indicating that governor-moxibustioncan improve learning and memo-ry ability of SAMP8 mice,and the curative effect is better than that of Chinese herbal medicine group(P<0.05 or P<0.01);the results of H.E. staining showed that compared with the normal group,the necrosis of the cells in the CA1 and CA3 regions was observed in the model mice;compared with the model group,the number of cell necrosis in the CA1 and CA3 areas in the governor-moxibustion plus Chinese herbal medicine group was the least,with the lightest morphological changes,while in the Chinese herbal medicine group,the injury was more obvious,indicating that the governor-moxibustion can protect hippocampal CA1 and CA3 neurons in SAMP8 mice,and can reduce cell damage. Conclusion:Governor-moxibustioncan improve the learning and memory ability of SAMP8 mice. The mechanism may be related to governor-moxibustion protecting neurons in hippocampal CA1 and CA3 area and inhibiting cell damage.