Effects of Qinggan Xiehuo Prescription on Expression of Transcription Factors in Th17 Cells of Graves' Disease Model Mice

GUO Yi

Abstract：Objective:To observe the effect of Qinggan Xiehuo prescription on the expression of transcription factors related to Th17 cells of Graves'disease(GD) model mice,investigate its mechanism in treating GD from the aspect of immune response and explore a new target in the treatment of GD with Qinggan Xiehuo prescription. Methods:A total of 80 female BALB/c mice were selected for the experiment. Among them,60 were chosen to construct the GD mouse model by using the recombinant adenovirus expressing the TSH re-ceptor A-subunit(TSHR-A). After the models were established,by the random number table method,the 60 mice models were divided into three groups:the model group,the methimazole group and the Qinggan Xiehuo prescription group,with 20 mice in each group. The other 20 mice in the 80 served as the control group. Mice in the methimazole group were given methimazole suspension by gavage,12 mg/kg a day. Mice in the Qinggan Xiehuo prescription group were given Qinggan Xiehuo prescription suspension,2 mL a day. Mice in the control group and the model group were given distilled water,2 mL a day. All the mice were intervened for 30 days. The levels of serum free triiodothyronine (FT3),free thyroxine (FT4) and thyroid stimulating hor mone(TSH) of mice in each group were measured by radioimmunoassay(RIA). The levels of serum inter-leukin-17 (IL-17) and interleukin-21 (IL-21) of mice in each group were examined by enzyme-linked im-munosorbent assay(ELISA). Results:Both methimazole and Qinggan Xiehuo prescription can reduce the ex-pression levels of serum FT3,FT4,TSH,IL-17 and IL-21. Compared with the model group,the differences were statistically significant(P<0.05). Conclusion:For the GD model mice,Qinggan Xiehuo prescription can inhibit the immune and inflammatory response of the thyroid gland and promote the recovery of thyroid function by inhibiting the expression of transcription factors related to Th17 cell.