Influence of Herb-Partition Moxibustion at Navel on Expression of VEGF and AMH in Rats with Premature Ovarian Failure

LU Yan; SHEN Qingsi; TIAN Meng; ZHANG Jing; ZHANG Yongchen; JIA Hongling

Abstract：Objective:To investigate the mechanism of herb-partition moxibustion at navel on vascular en-dothelial growth factor (VEGF) and anti-mullerian hormone (AMH) in rats with premature ovarian failure (POF). Methods:Fifty female Wistar rats were randomly divided into normal group,model group,herb-parti-tion moxibustion at navel group and western medicine group. The rat model of POF was established by in-traperitoneal injection of cyclophosphamide. The rats in the herb-partition moxibustion at navel group were treated with self-made herb ointment. The rats in the western medicine group were given estradiol valerate by gavage. After 2 weeks of treatment,the levels of E2,LH and FSH were detected by using the radioim-munoassay method. Enzyme-linked immunosorbent assay(ELISA) was used to detect the level of VEGF and AMH. Results:Compared with the normal group,the level of E2 decreased in the model group(P<0.05). The level of E2 in the herb-partition moxibustion at navel group and western medicine group was higher than that in the model group(P<0.05). Compared with the normal group,the level of VEGF and AMH signif-icantly decreased in the model group(P<0.05). The level of VEGF and AMH in the herb-partition moxibus-tion at navel group and western medicine group increased,compared with the model group(P<0.05). There was no statistical difference of the level of VEGF and AMH between the herb-partition moxibustion at navel group and western medicine group (P>0.05). Conclusion:The herb-partition moxibustion at navel can significantly promote the follicle number and ovari-an reserve of POF rats,which may be related to the its regulation on E2,VEGF and AMH.