SDS-PAGE Analysis for Proteins in Coix Seed
CUI Xueying
XIANG Meirong
WU Hao
RONG Rong
JIANG Haiqiang
Abstract：Objective:To compare the differences of protein categories before and after acid-isolation and to study the protein molecular weights in Coix seed. Methods:An alkali-solution and acid-isolation method was employed to extract the proteins in Coix seed,and the protein was isolated by SDS-PAGE analysis,and the molecular weights were calculated by the standard curve method. Results:There were 6 bands of soluble protein , and the molecular weights were determined as follows:91 . 598 , 77 . 169 , 39 . 839 , 28 . 977 , 23 . 822 and 1 6.1 kDa. Proteins in Coix seed before and after acid-isolation were compared and it was found that two protein bands,23.822 kDa and 16.1 kDa,were missing. Conclusion:Through this method,the resulted spectro-gram was clear with good repeatability,and could guide the isolation and analysis of proteins in Coix seed.
Keywords：proteins in Coix seedSDS-PAGEmolecular weights
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：3( 155-157 )