Ultrasonic Extraction Technology for Total Flavonoids from Radix Sophorae Flavescentis and Its Antimicrobial Activity
FANG Kang
CHEN Chen
GAO Rongkai
WANG Suyun
ZHANG Yingying
ZHAO Qitao
YAN Bin
Abstract：Objective:To explore the optimized ultrasonic extraction condition for total flavonoids in Radix Sophorae Flavescentis and the minimal inhibitory concentration (MIC) for various pathogenic bacteria. Meth ods:L18 (37) orthogonal experimental method was used to determine the optimized ultrasonic extraction tech-nology,and MIC was determined through micro-dilution method. Results:The optimized ultrasonic extraction conditions were as follows:ratio of material to solution was 1∶10;50% ethanol concentration,15 minutes for extraction,pH value 9,and extracted for 1 time. MIC test showed that the total flavonoids from Radix Sophorae Flavescentis had certain inhibitory effect on various pathogenic bacteria. Conclusion:Ultrasonic ex-traction method was fast,easy to operate,and energy saving. This process can be used to extract the total flavonoids from Radix Sophorae Flavescentis in laboratory. Total flavonoids from Radix Sophorae Flavescentis showed certain inhibitory effect on Staphylococcus aureus,Escherichia coli,Bacillus subtilis,Klebsiella pneu-monia,but the effect on Pseudomonas aeruginosa was not obvious.
Keywords：total flavonoids from Sophorae Flavescentis Radixultrasonic extractionorthogonal experimenttechnology researchpathogenic bacteria
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：3( 989-991 )