Experimental Study of Feiyiliu Mixture on Inhibiting the Growth of Lung Cancer A549 Xenograft Tumor in Nude Mice
CAO Yufeng
SONG Haiyan
SUN Qinglan
WANG Suling
ZHEN Xin
Abstract：Objectives:To observe the effect of Feiyiliu mixture on inhibiting lung cancer A549 xenograft tu-mor innude mice and to investigate the underlying mechanisms. Method:Nude micewere transplanted with lung cancer A549 cell line,and weredivided into the control group,Feiyiliu mixture group,and cisplatin group. The effect of Feiyiliu mixture on inhibiting the growth of xenograft tumor was observed. Epidermal growth factor receptor(EGFR) mRNA levelwas detected by real-time PCR,andEGFR,P38,GSK3β and AKT levels were detected withwestern blot assay. Results:Feiyiliu mixture had significant effects of inhibiting tu mor growth(P<0.05).Compared with the control group,EGFR mRNA level in the tumor tissues in both the Feiyiliu mixture group and cisplatin group was down-regulated(P<0.05),and the protein level of EGFR and p-EGFR also showed a trend of decreasing,but without statistical significance;protein level of P38,GSK3βand AKT showed no significant changes,but the protein levels of p-P38,p-GSK3β and p-AKTwere signifi-cantly down-regulated(P<0.05). Conclusion:Feiyiliu mixture couldinhibit the growth of lung cancer A549 xenograft tumor in nude mice,possibly through inhibiting phosphorylation levels of protein P38,GSK3β and AKT to restrain tumor growth.
Keywords：Feiyiliu mixtureA549lung cancercisplatinxenograft tumorinhibition
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 901-904 )