Determination of Carboxyatractyloside and Atractyloside in Xanthii Fructus and its Preparation with High Performance Liquid Chromatography Evaporative Light-Scattering Detector
GUO Dongxiao
WANG Bing
LIN Lin
LIU Hongchao
CUI Weiliang
XU Lihua
LIN Yongqiang
Abstract：Objective:To develop a high Performance liquid chromatography evaporative light-scattering detec-tor (HPLC-ELSD) for determining contents of two poisonous components ,carboxyatractyloside and atractyloside in Cangerzi(Xanthii Fructus) and its preparation(Xinqin tablets and Xinqin granules). Method:A Kromasil 100-5C18 column(4.6 mm×250 mm,5μm) with the mobile phase consisting of acetonitrile(A)-10 mmol/L and ammonium acetate solution(B),in gradient elution manner(0~30 min,12%A→20%A;30~31 min,20%A→12%A;31~35 min,12%A). The column temperature was 35℃ and the flow rate was 1.0 mL/min. The drift tube temperature of ELSD was set at 95℃ with the N2 flow rate at 1.6 L/min. Result:Carboxyatractyloside and a-tractyloside exhibit good linearity within the ranges from 0.681 to 4.086μg and from 0.731 to 4.383μg,re-spectively. The regression equations were Y=1.4973X+3.9461 (r=0.9996) and Y=1.4619X+4.0630 (r=0.9991);the average recovery rate(n=6) was 96.4%(RSD=1.2%) and 97.7%(RSD=0.9%),respectively. Conclusion:The established HPLC-ELSD method was simple and repeatable,and it could be applied to the quality control of Xanthii Fructus and its preparation.
Keywords：high performance liquid chromatography ( HPLC )evaporative light-Scattering detector ( ELSD )Xanthii FructuXinqin tabletsXinqin granulescarboxyatractylosideatractyloside
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 64-67 )