Ultraviolet-Visible Spectroscopy and Thin-Layer Chromatography Identification of Stems and Leaves of Scutellaria Baicalensis Georgi
XU Depeng
ZHANG Wenling
SHI Junying
Abstract:Objective:To identify stems and leaves of Scutellaria baicalensis georgi by ultraviolet-visible spec-troscopy(UV-visible spectra) and thin-layer chromatography(TLC). Methods:The extracts of petroleum ether, chloroform,ethanol and water of stems and leaves of Scutellaria baicalensis georgi were scanned by UV-visi-ble spectra respectively. The number and position of the absorption peaks were also determined. With polyamide and silica gel G as adsorbent ,the TLC of stems and leaves of Scutellaria baicalensis georgi was done and the separation of its components were observed. Results:In UV-visible spectra,extracting same sample from different extraction solvent can get different number and position of absorption peaks;in TLC,all different adsorbents and developing solvents can make a good separation of components of Scutellaria baicalensis georgi. Conclusion:Both the two methods are simple,accurate and sensitive,so they can be used for rapid identification of stems and leaves of Scutellaria baicalensis georgi.
Keywords:stems and leaves of Scutellaria baicalensis georgiultraviolet-visible spectroscopy (UV-visible spectra)thin-layer chromatography(TLC)
Publication Date:2015-01-01
Online Publishing Date:2026-05-22(First online date of this platform, not the publication date of the document)
Pages:3( 284-286 )
