Optimization of Ultrasonic Extraction Parameters and Deprotein Process for Polysaccharide from Sijunzi Decoction
ZHOU Shuaifei
LIU Yuhong
Abstract：Objective:To optimize the parameters of ultrasonic extraction and deprotein process for polysac-charide of sijunzi decoction. Method:With the extraction temperature,extraction time,extraction times as three factors,orthogonal experiment of three levels were designed to optimize the parameters of ultrasonic extraction for polysaccharide of sijunzi decoction. Sevag method and Sevag-enzyme method were compared for deprotein of polysaccharide of sijunzi decoction,and single-factor experiments were designed. Result:The optimal condi-tions were that polysaccharide of sijunzi decoction was extracted at 80°C,30 min for 3 times. Sevag-enzyme method was better for deprotein than Sevag method, and the optimum condition was that the amount of trypsin was 1%,with water bath at 37℃,enzymolysis for 3 hr,and processed with Sevag agent for 3 times. Conclusion:The parameters of ultrasonic extraction and deprotein process for polysaccharide of sijunzi decoc-tion were optimized,and it could be the basis for the further isolation and activity study.
Keywords：Sijunzi decoctionpolysaccharideultrasonic extractiondeprotein
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：3( 134-136 )