Study of Lagopsis Supina in Vitro Anti-herpes Simplex Virus
WANG Xu
YAO Meiyue
KONG Jing
PANGRong
CUIYanxia
ZHOU Changzheng
Abstract：Objective:To explore crude extracts with different polarity from the Lagopsis supina in vitro inhi-biting herpes simplex virus type I ( HSV-1 ) , and to do the preparation for isolating the active ingredi-ents for anti-HSV-1 by further purifying. Methods:Pure water,95% ethanol,ethyl acetate were used as the solvent to extract drugs. Hep-2 cells were infected with HSV-1 in vitro to evalute anti-HSV-1 effect. The al-cohol extract was further purified by precipitation with water ,and sediment and the supernatant were tested for the in vitro antiviral experiments respectively. The cytopathic effect (CPE) was observed under a micro-scope. Neutral red staining was measured in a microplate reader at 540 nm to calculate the therapeutic in-dex(TI). Results:Among the three total extracts from Lagopsis supina,the ethanol total extract is best ef-fect. After alcohol total extract was precipitated with water ,the supernatant index TI was greater than 46.5 , and the sediment TI index was greater than 51.6. Conclusion:Total alcohol extract from Lagopsis supina has anti-HSV-1 effects. After processed with ethanol extraction and water precipitation ,its supernatant and sedi-ment have obvious anti-HSV-1 effects.
Keywords：Lagopsis supinatotal extractethanol extraction and water precipitationherpes simplex virusan-tiviral
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 928-931 )