Determination of Ursolic Acid in Scutellaria barbata D. Don through High Performance Liquid Chromatography
YIN Wenjie
LIU Yanqun
HUANG Zhugang
YU Liang
LV Qingtao
JIANG Haiqiang
GONG Lili
Abstract：Objective:To determine ursolic acid in Scutellaria barbata D . Don using high performance liquid chromatography ( HPLC ) . Methods:Agilent Eclipse SB-C18 column ( 5 μm , 4 . 6 mm × 250 mm ) was adopted with a mobile phase of methanol-2% Acetic Acid(88∶12) at the flow rate was 1.0 mL/min,the detection wavelength was set at 210 nm and the column temperature was 30 ℃. Results:The content of ursolic acid was linear in the ranges of 0.0245~0.4918 mg/mL(r=0.9996) for ursolic acid. The average recoveries(n=3) of ursolic acid were 98.7%(RSD=1.90%). The sample solution was steady within 24 h. Conclusion:This method is precise and accurate for content determination of ursolic acid in Scutellaria barbata D.Don.
Keywords：Scutellaria barbataursolic acidhigh performance liquid Chromatography(HPLC)content determi-nation
Publication Date：2014-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：3( 582-584 )