Effect of Ezhu Power on Expressions of Cyclin A and P21 in Endometriotic Tissues of Rats with Endometriosis

GE Huijuan; CAO Baoli

Abstract：Objective:To observe the differences of Cyclin A and P21 expressions in eutopic and ectopic tissues of rats with endometriosis and explore the mechanism of compound Ezhu powder to treat endometrio-sis from the aspect of the cell cycle regulation. Methods:The rat model with endometriosis was established with self-endometrium transplantation. The established rat models were divided into five groups:compound Ezhu power high dosage group,compound Ezhu low dosage group,gestrinone control group,model control group,and the normal control group, given the gavage of 1 mL/100 g. After the treatment,the expressions of Cyclin A and P21 of in eutopic and ectopic tissues were tested with immuno-histochemistry method. Re-sults:The expression of Cyclin A in endometriotic tissues was significantly higher than that in normal en-dometrial tissues,whereas the expression of P21 in endometriotic tissues was significantly lower than that in normal endometrial tissues. There was negative correlation between expression of P21 and Cyclin A in en-dometriosis. Compound Ezhu power inhibited the growth of endometriotic tissue in rat model ,decreased the volume of ectopic focus,suppressed expression of Cyclin A,and enhanced the expression of P21,which obvi-ous statistical difference compared with the model group (P<0.05). Conclusion:Compound Ezhu power could inhibit the expression of Cyclin A,and increase the expression of P21. From the aspect of cell cycle regula-tion,it is confirmed that the compound Ezhu power has a regulatory effect on endometriosis,and such regu-latory effectmay be one of the mechanism to treat endometriosis.