Inhibitory effect of α-glucosidase by Eleutherococcus senticosus extract and enzyme kinetics analysis
YAN Lin
NI Tianfeng
CHEN Xiaoqiang
ZHANG Ying
Abstract：Objective To investigate the inhibitory effect and underlying mechanism of Eleutherococcus senticosus ex-tract on α-glucosidase.Methods The half-maximal inhibitory concentration(IC50)was determined using in vitro enzyme inhibition assays.Enzyme kinetics and fluorescence spectroscopy were employed to analyze the inhibition type,interaction mechanism,and thermodynamic parameters.Results E.senticosus extract exhibited a dose-dependent inhibitory effect on α-glucosidase,with an IC50 value of 0.86 mg·mL-1.The inhibition was characterized as predominantly mixed-type with a non-competitive component.Fluorescence spectroscopy revealed that the extract induced conformational changes in the enzyme through dynamic quenching.Thermodynamic analysis indicated that hydrophobic interactions were the main driving force.Conclusion E.senticosus extract inhibits α-glucosidase through conformational modulation and hydrophobic interactions,suggesting its potential application in blood glucose regulation.
Keywords：Eleutherococcus senticosusα-glucosidaseKinetic analysisFluorescence spectroscopyHypoglycemic activ-ity
Publication Date：2026-02-28
Online Publishing Date：2026-03-25(First online date of this platform, not the publication date of the document)
Pages：7( 134-140 )