Inhibitory activity of jaceosidin on the SARS-CoV-2 RNA polymerase
DI Kun
LIU Qi
WU Qiaona
LIU Jinglei
WANG Yujia
SU Yanlei
SUN Dianxing
ZHAO Jianyuan
Abstract：Objective To explore the inhibitory activity of the flavonoid jaceosidin against the RNA polymerase of the novel coronavirus.Methods Molecular docking was first employed to investigate the binding mode of jaceosidin at the active site of RNA polymerase,to determine whether jaceosidin could stably bind to the NTPs channel and obstruct the entry of substrates.Subsequently,the inhibitory pharmacological activity of jaceosidin against RNA polymerase was evaluated using a cell-based assay system for the catalytic reaction of the novel coronavirus RNA polymerase.Results Jaceosidin exhibited favorable binding to the active site of RNA polymerase,occupying the NTPs channel and hindering the entry of nucleotides,thereby achieving pharmacological action to inhibit RNA polymerase-mediated nucleic acid replication.The results of pharmacological experiments at the cellular level demonstrated that jaceosidin possesses inhibitory activity against RNA polymerase,albeit with weaker activity compared to remdesivir.However,jaceosidin exhibited lower cytotoxicity compared to remdesivir.Conclusion The findings of this study indicate that jaceosidin exhibits inhibitory activity against RNA polymerase,providing a potential reference for the development of anti-novel coronavirus drugs.
Keywords：SARS-CoV-2RNA polymeraseActive siteJaceosidinMolecular dockActivity test
Publication Date：2026-02-28
Online Publishing Date：2026-03-25(First online date of this platform, not the publication date of the document)
Pages：5( 130-133,149 )