Study of Chidan Tuihuang Granules alleviating ANIT-induced cholestasis by inhibiting apoptosis signaling pathway
ZENG Lingshun
SHI Senpan
WANG Yile
TAN Lingzhi
TAN Xiyue
FU Lingping
MA Xiao
Abstract：Objective To explore the mechanisms by which Chidan Tuihuang Granules(CDTH)improve cholestatic liver injury and to reveal the biological mechanisms that regulate the progression of cholestasis.Methods Using α-naphthyl isothiocyanote(ANIT)-induced cholestasis model,the therapeutic effects of CDTH on cholestasis were evaluated.Network pharmacology and molecular docking techniques were used to identify potential core targets and pathways for CDTH in treating cholestasis.Results Animal experiments confirmed that CDTH significantly reduced liver function indicators and effectively improved liver tissue inflammation and steatosis.Network pharmacology analysis showed that CDTH treatment of cholestasis was significantly enriched in apoptosis signaling pathways.Molecular docking analysis confirmed that the active components of Chidan Tuihuang Granules have a stable binding ability with core targets(binding energy＜-5.0 kcal·mol-1).Conclusion CDTH can effectively improve serum biochemical indicators of ANIT-induced cholestasis,inhibit the expression of apoptosis signaling pathway protein in cholestatic of liver tissue,and thus exert a comprehensive therapeutic effect against cholestasis.
Keywords：Chidan Tuihuang GranulesCholestasisMolecular dockingNetwork pharmacologyApoptosis
Publication Date：2025-12-30
Online Publishing Date：2026-01-22(First online date of this platform, not the publication date of the document)
Pages：9( 1145-1152,1179 )