Exploring the material basis and mechanisms of Rubus suavissimus S.Lee against diabetic nephropathy through network pharmacology and in vivo experiments
WANG Puyu
SUN Shuqing
ZHENG Hua
Abstract：Objective To explore the material basis and mechanism of Yaoshan sweet tea(Rubus suavissimus S.Lee,RS)in treating diabetic nephropathy.Methods UPLC-Q-TOF-MS was used to analyze the components of the water ex-tract of Yaoshan sweet tea,the database was used to search for potential targets for diabetes nephropathy,construct a protein interaction network,and enrich GO and KEGG pathways.Molecular docking and animal experiments were carried out to verify the screened core components and core targets.Results A total of 47 chemical components and 744 potential active ingredient targets were identified in RS,along with 372 diabetic nephropathy targets.Two key components,kaempferol and quercetin,were screened out,along with six core targets:TP53,SRC,STAT3,PIK3CA,PIK3R1,and HSP90AA1.KEGG pathway enrichment analysis revealed that the PI3K-Akt signaling pathway,MAPK pathway,and others were closely associ-ated with diabetic nephropathy.Molecular docking results and animal experiments demonstrated that kaempferol exhibited strong binding affinity with the core target PIK3R1.Additionally,it effectively reduced blood glucose and urea nitrogen levels in diabetic nephropathy rats,while alleviating renal tubular dilation and glomerular fibrosis in the model group.Conclusion Kaempferol may serve as the primary active component of RS against DN,potentially exerting therapeutic effects by regu-lating autophagy-and mitophagy-related proteins in PI3K-Akt and MAPK signaling pathways.
Keywords：Rubus suavissmus S.LeeDiabetic nephropathyNetwork pharmacologyUPLC-Q-TOF-MSKaempferol
Publication Date：2025-11-30
Online Publishing Date：2025-12-24(First online date of this platform, not the publication date of the document)
Pages：9( 1056-1063,1138 )