Study on the terpenoid composition in the stem of Tripterygium wilfordii
ZHANG Qiuyang
TIAN Yulu
ZHANG Lingzhi
SUN Hua
SU Guangyue
ZHANG Dongming
Abstract：Objective To study the chemical constituents of the stems of Tripterygium wilfordii Hook.f.(Celastraceae).Methods Thin layer chromatography,ODS column chromatography and high-performance preparative liquid chromatogra-phy(HPLC)were used to separate and purify the 80％ethanol extract of T.wilfordii stems,and the structures of the com-pounds were identified by mass spectrometry(MS),nuclear magnetic resonance(NMR)and other spectroscopic methods.Results Eleven terpenoids were isolated from an 80％ethanol extract of T.wilfordii,including two diterpenoids:triptonoter-pene(1),dehydroabietic acid(2);Nine triterpenoids:betulinic acid(3),epi-oleanolic acid(4),3-epiursolic acid(5),codopitirol A(6),oleanoic acid(7),betulonic acid(8),3α-hydroxy-23-oxoolean-12-en-28-oic acid(9),oleanonic acid(10),ursonic acid(11).Conclusion Compounds 4～6,8,9,and 11 were first isolated from T.wilfordii.The isolated compounds 1～9 were evaluated for their hepatocyte injury protective activity,and the results showed that compounds 2～9 had significant protective effects against paracetamol-induced HepG2 cell injury.
Keywords：Tripterygium wilfordiiTerpenoidsChemical constituentsHepatoprotective activity
Publication Date：2025-10-30
Online Publishing Date：2025-11-26(First online date of this platform, not the publication date of the document)
Pages：6( 955-960 )