Rapid screening of 4 steine expectorants illegally added to traditional Chinese medicine and healthy food by UPLC-MS/MS
YU Haibo
ZHANG Dandan
CHEN Hui
Abstract：Objective To establish a UPLC-MS/MS method for rapidly detecting the illegal addition of 4 steine expectorants(carbocysteine,fudosteine,mecysteine hydrochloride,erdosteine)in traditional Chinese medicine and healthy food.Methods The chromatography was separated on a Thermo Hypersil GOLD aQ C18 column(2.1 mm×100 mum,1.9μm)with 10 mmol·L-1 ammonium formate solution(containing 0.1％formic acid)-methanol as the mobile phase.Gradient elution was performed at column temperature of 45 ℃ and flow rate of 0.25 mL·min-1.Electrospray ion source(ESI)positive ion mode and multiple reaction monitoring(MRM)were used for mass spectrometry detection.Results The four chemical components showed good linear relationship in a certain range and obtained good separation.The average recovery of low,medium and high level illegal added concentrations was 89.5％to 109.2％,RSD was 0.29％to 3.51％,and the detected concentration was 0.45～3.27 ng·mL-1.Conclusion The method was accurate,rapid and specific,and can be applied to the detection of illegal addition of steine expectorants,and provides technical support for the daily supervision of market supervision departments.
Keywords：UPLC-triple quadrupole mass spectroscopySteine expectorantsIllegal addition
Publication Date：2025-09-30
Online Publishing Date：2025-10-30(First online date of this platform, not the publication date of the document)
Pages：4( 874-876,920 )