The effects of Qiwei Baizhu San on the mRNA expression of enteric neuron cell bodies in type 2 diabetic rats based on transcriptomics

QI Yunwen; GUO Liangqing; HAN Xiaochun

doi:10.13506/j.cnki.jpr.2025.09.004

Abstract：Objective To investigate the effect of Qiwei Baizhu San on the mRNA of enteric neuron cell bodies in type 2 diabetes rat model based on high-throughput transcriptome sequencing.Methods The type 2 diabetes rat model was es-tablished by injection of Streptozocin(STZ)combined with high-fat and high-sugar diet,and the rats were divided into control group,model group and Qiwei Baizhu San treatment group.3 rats in each group were randomly selected for intestinal mRNA sequencing.Gene ontology(GO)enrichment analysis was performed on the model and control groups.Differentially expressed genes were identified based on the function of neuron cell bodies in the cellular component.The annotated 3 groups of differentially expressed genes were analyzed using IBM SPSS Statistics 25 software for principal component analysis(PCA)and clustering analysis,and Bi analysis was conducted on the loadings and scores of the principal compo-nent analysis.Results There were 13 differentially expressed genes in the function of neuron cell bodies between the model rats and the control rats.The Bi analysis of the loadings and scores of the principal component analysis of the differentially expressed genes showed a clear distinction between the model group and the control group,consistent with the Results of the sample clustering.After normalization of PC1,PC2,and PC3,it was found that the weights of the genes were different,and the top 10 genes by weight were Plxdc1,Sez6l,Adora1,Trpm5,Trpm2,Ncf1,Lrrk2,Cd40,Klhl14,and Cdk5r1.These genes were identified as key targets for the treatment of type 2 diabetes in rats by Qiwei Baizhu San via enteric neuron cell bodies.Conclusion Qiwei Baizhu San can ameliorate the damage of enteric neuron cell bodies induced by modeling stimulation,and maintain the normal function of enteric neuron cell bodies by up-regulating the mRNA expression levels of Plxdc1,Sez6l,Adora1,Trpm5,Trpm2,Ncf1,Lrrk2,Cd40,Klhl14,and Cdk5r1.It can regulate the balance of glucose metabolism to maintain the enteric homeostasis and effectively control the progression of type 2 diabetes mellitus.