Determination of 8 components in Lonicera japonica Formula Granules by two reference substances for determination of multiple components method
BA Ranran
LIU Fei
GENG Feng
ZHANG Jing
WANG Xuchen
JI Guoli
LIU Yuling
Abstract：Objective To establish a two reference substances for determination of multiple components method for 8 components in Lonicera japonica Formula Granules.Methods HPLC method was used,with chlorogenic acid and 3,5-O-dicaffeoylquinic acid as the reference substances for the determination.The position of each component's chromatographic peak was determined by linear calibration using two reference substances;The retention time of unknown components was predicted,and the method was validated.The relative correction factor of each component was calculated based on chlorogenic acid as the reference,and the components were quantified.Results The linear calibration using two reference substances can predict the retention time of unknown components on unknown chromatographic columns more accurately than the relative retention time method.The RSD of the relative correction factor method is less than 2％compared with the content determination by external standard method.Conclusion The two reference substances for determination of multiple component method for determining 8 components in Lonicera japonica Formula Granules is feasible and accurate.
Keywords：Lonicera japonica Formula GranulesTwo reference substances for determination of multiple component methodLinear calibration using two reference substancesRelative correction factor
Publication Date：2025-08-30
Online Publishing Date：2025-09-30(First online date of this platform, not the publication date of the document)
Pages：6( 762-766,773 )