Characterization of the compounds of Hedyotis diffusa alcohol extract based on UPLC-QTOF-MS and its spectral effect relationship of liver cancer
YU Jiaxin
ZHANG Qiang
MENG Xiansheng
Abstract：Objective To elucidate the substance basis of Hedyotis diffusa alcohol extract for anti liver cancer through UPLC-QTOF-MS technology and spectral correlation analysis.Methods HPLC was used to establish ten batches of fingerprint spectra of Hedyotis diffusa alcohol extract,and the anti liver cancer efficacy of Hedyotis diffusa alcohol extract was analyzed using the proliferation inhibition rate and apoptosis necrosis rate of liver cancer cell line SMMC-7721 as indicators.Further analyze the spectrum effect correlation of Hedyotis diffusa alcohol extract against liver cancer using grey relational analysis and partial least squares analysis.Finally,UPLC-QTOF-MS technology was used to identify the common peaks.Results Hedyotis diffusa alcohol extract significantly inhibited the proliferation and induced apoptosis of liver cancer cell line SMMC-7721,with an IC50 of 3.42 mg·mL-1.Spectral analysis shows that peaks 12,4,44,9,11,7,and 10 are positively correlated,while peaks 2,38,27,and 29 are negatively correlated.Among them,peak 12 is 5-caffeoylquinic acid,peak 4 is 4-OD-p-glucopyranosyl-p-coumaric acid,peak 9 is tachiogroside B,and peak 2 is succinic acid.Conclusion The Hedyotis diffusa alcohol extract could inhibit the proliferation of liver cancer cells,and its material basis may be 5-caffeoylquinic acid,4-OD-glucopyranosyl-p-coumaric acid,tachiogroside B,etc.
Keywords：Hedyotis diffusaSpectral efficiency correlationLiver cancerMaterial basisUPLC-QTOF-MS
Publication Date：2025-07-30
Online Publishing Date：2025-08-21(First online date of this platform, not the publication date of the document)
Pages：7( 649-654,673 )