Synthesis of gem-difluoromethylenated oleanolic acid and its anti-hepatic fibrosis effect
WANG Juan
ZHENG Xing
ZHANG Si
WANG Guan
DAI Ling
YU Yingzi
YAO Xu
ZHENG Zitong
Abstract：Objective To assess the protective efficacy of the novel compound difluoromethylenated oleanolic acid(DFOA)against liver fibrosis injury in mice,induced by carbon tetrachloride(CCl4).Methods To prepare DFOA and confirm its structure,84 mice were divided into normal control,liver fibrosis model,and low,medium,and high-dose DFOA groups,as well as a positive control group.Liver fibrosis was induced using CCl4,and the mice were administered drugs by gavage for 4 weeks.Serum liver function indicators(ALT,AST,ALB)were tested,and histopathological analysis of liver tis-sue was performed.Results Treatment with DFOA led to a statistically significant decrease in ALT and AST levels in mice with liver fibrosis,accompanied by a notable increase in ALB levels.Compared to the model group,DFOA-treated mice ex-hibited marked improvements in liver injury and a reduced extent of fibrosis.Conclusion DFOA emerges as a promising a-gent with a notable protective effect against CCl4-induced liver fibrosis,highlighting its potential applications in hepatopro-tection,transaminase reduction,and fibrosis antagonism.
Keywords：Oleanolic acidMiceGem-difluoromethylenated oleanolic acidHepatic fibrosis
Publication Date：2025-06-30
Online Publishing Date：2025-08-21(First online date of this platform, not the publication date of the document)
Pages：4( 543-546 )