Effect and mechanism of Musa basjoo Anshen formula on sleep improvement in mice

TANG Huiqin; LU Yiping; LIU Yuanyang; LI Yuqiang; GUO Qiaoqin; ZEN Chunhui; ZHANG Yu; LIN Qunfeng; YANG Ke

doi:10.13506/j.cnki.jpr.2025.06.003

Abstract：Objective To study the sedative effect and mechanism of Musa basjoo Anshen formula on sleep improve-ment in mice.Methods Normal mice,synergistic subthreshold,and sleep dose of Urethane were used to observe the effects of Musa basjoo Anshen formula on spontaneous activity,sleep rate,sleep latency,and sleep duration on mice;The PCPA-in-duced insomnia mouse model was tested by elevated cross maze test and open field test,and the contents of 5-HT,GABA and GLU in the brain tissue were detected.The protein expression levels of HTR1A,GABRG2 and GABRA1 were deter-mined by Western blotting technique.Results Musa basjoo Anshen formula had no significant effect on the autonomic activ-ity of normal mice(P＞0.05).The number of sleeping animals and sleeping rate of mice with subthreshold dose of Urethane were significantly increased(P＜0.05).The number of open arms and the percentage of stay time of PCPA-induced insomnia mice entering the elevated cross maze were significantly increased(P＜0.05,P＜0.01).The upright times of PCPA-induced insomnia mice in open field were significantly increased(P＜0.01),and the modification times were significantly decreased(P＜0.05).The results showed that Musa basjoo Anshen formula could significantly increase the contents of 5-HT and GA-BA in the brain tissues of PCPA-induced insomnia mice(P＜0.05),and significantly decrease the content of GLU in the brain tissues of PCPA-induced insomnia mice(P＜0.05).Western blotting detection showed that the protein expression of HTR1A,GABRG2 and GABRA1 could be significantly increased by Musa basjoo Anshen formula(P＜0.05).Conclusion Musa basjoo Anshen formula can regulate the expression of sleep-related HTR1A,GABRG2 and GABRA1 proteins,improve sleep and anxiety in mice,and have sedative and tranquilizing effects.