Qualitative determination of multiple components in Angelica sinensis by linear calibration using two reference substances
LUAN Yongfu
LIU Hongchao
LIN Xiao
MA Shuangcheng
SUN Lei
LIU Xiaoyan
LIN Yongqiang
WANG Bing
Abstract：Objective To establish an HPLC method for qualitative determination of chlorogenic acid,ferulic acid,sen-kyunolide I,coniferyl ferulate and ligustilide in Angelica sinensis,in order to detect the feasibility of linear calibration using two reference substances(LCTRC).And the two alternative qualitative methods of reference are compared between LCTRC and the relative retention time method(RRT).Methods HPLC method was adopted,the retention time of the 5 compo-nents in Angelica sinensis were determined on 19 C18 columns of different brands or types.The chromatographic peak was i-dentified by retention time of reference.The standard retention time(SRT)was calculated.Both chlorogenic acid and ligusti-lide were taken as the two reference substances to forecast the retention time of the other three components by LCTRC.An-other 2 C18 column was used for methodology validation and sample determination.Senkyunolide I was used as the reference substance to forecast the retention time of the other 4 components by RRT.Results Chlorogenic acid,ferulic acid,sen-kyunolide I,coniferyl ferulate and ligustilide were qualitatively analyzed by LCTRC.The retention time of the 5 components on 19 chromatographic columns had good linear relationship(r＞0.995),and the SRT of them was 9.761,14.866,17.942,28.304 and 34.277 min respectively.LCTRC is more accurate and suitable for more chromatographic columns than RRT.Conclusion As a new alternative reference method,LCTRC can be used to assist the chromatographic peak identification,worthy of application and promotion.
Keywords：Angelica sinensisHPLCLCTRCRRTSRTChromatographic peak identification
Publication Date：2025-05-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 457-462 )