Comprehensive evaluation of the quality of Yunnan Baiyao using multi-component quantitative analysis combined with chemometrics and entropy weight TOPSIS method
XING Qimei
CHEN Xueting
LIN Long
ZHENG Yanfang
HUANG Mingqing
Abstract：Objective To evaluate the quality of Yunnan Baiyao using multi-component quantitative analysis combined with chemometrics and the entropy weight technique for order preference by similarity to ideal solution(TOPSIS).Methods The content of notoginsenoside R1,ginsenoside Re,Rg1,F3,Rg2,Rb1,F1,Rb2,Rb3,Rd,F2,Rg5,20(S)-Rg3,and 20(R)-Rg3 in Yunnan Baiyao was determined using ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry(UPLC-MS/MS).Chemometrics and entropy weight TOPSIS were applied to evaluate the quality of 22 batches of Yunnan Baiyao and Yunnan Baiyao Capsules.Results The components showed a good linear relationship within a certain concentration range(r ≥0.995 2),with good precision,repeatability,and stability.The recovery rate of added samples was 94.6％～105.4％,and the relative standard deviation(RSD)was 0.97％～5.6％.Based on the chemometric analysis and the en-tropy weight TOPSIS method,the 22 sample batches were categorized into three groups.The variable importance projection(VIP)values for ginsenoside Rb1,ginsenoside Rg1,and notoginsenoside R,were all greater than 1.Sample S1 exhibited the highest quality.Conclusion This method enables rapid,sensitive,specific,and efficient quantitative analysis of 14 saponin components in Yunnan Baiyao and can be used for quality control of Yunnan Baiyao.
Keywords：Yunnan BaiyaoUltra-high performance liquid chromatography-tandem triple quadrupole mass spectrome-tryChemometricsEntropy weight TOPSIS methodSaponinsContent determination
Publication Date：2025-05-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 422-428,449 )