Study on the effect and mechanism of Tibetan medicine Rannasangpei in stabilizing atherosclerotic Plaques

LIU Lei; RONG Yicheng; LIU Xinyu; YANG Le; CHEN Liming

doi:10.13506/j.cnki.jpr.2025.04.005

Abstract：Objective To investigate the role and mechanism of the Tibetan medicine Rannasangpei(RNSP)in stabili-zing atherosclerotic plaques.Methods Male Apolipoprotein E-knocknout(ApoE-/-)mice aged 6～8 weeks were fed a high-fat diet to establish atherosclerotic models.ApoE-/-male mice fed with normal diet were taken as blank control group(n=10),and the established atherosclerotic models mice were randomly divided into model group(fed with high-fat diet,n=10),Atorvastatin group[10 mg·(kg·d)-1 atorvastatin administered intragas trically,n=10]and RNSP group[7 mg·(kg·d)-1 RNSP administered in tra gas trically,n=10].The atherosclerotic area of aorta,the cross-sectional area of aortic root plaque and the lipid composition in plaque were estimated by oil red O staining.Sirius red staining was used to e-valuate collagen composition.CD68 and α-SMA immunohistochemistry were used to quantitatively analyze the components of macrophages and smooth muscle,respectively.In addition,we calculated the plaque vulnerability index of different groups of mice.Results No significant difference in initial weight among model group,RNSP group and Atorvastatin group(P＞0.05).Compared with the model group,Atorvastatin group showed the expected blood lipid regulation effect,while RNSP group did not show significant blood lipid improvement;The aortic arch lesions in RNSP group and Atorvastatin group showed a significant remission trend;The area of atherosclerotic lesions in RNSP group and Atorvastatin group was signifi-cantly reduced(P＜0.05).Compared with the model group,RNSP and Atorvastatin groups significantly improved the struc-tural stability of plaque and significantly reduced the vulnerability index of plaque(P＜0.05).Conclusion The Tibetan medicine RNSP could exert its anti-atherosclerosis effect through multi-target regulation,providing new scientific basis and theoretical support for the clinical transformation of Tibetan medicine.