Analysis of chemical constituents of Chaenomeles speciosa and Chaenomeles sinensis based on UPLC-Q-Exactive Orbitrap-MS

FAN Lixiang; WANG Aiyuan; CHEN Jinshu; LIU Xiaoxi; CUI Xuyang; ZHANG Yongqing

doi:10.13506/j.cnki.jpr.2025.03.006

Abstract：Objective The aim of this study was to analyze the chemical compositions of Chaenomeles speciosa and Chaenomeles sinensisusing UPLC-Q-Exactive Orbitrap-MS and compare the composition differences of two species.Methods The separation was performed on a Halo C18 column(2.1 mm×100 mm,2.7 μm)with a gradient elution of 0.05％formic acid(A)-formic acid acetonitrile(B)as the mobile phase.Flow rate was 0.3 mL·min-1 and column temper-ature was 40℃with an injection volume 3 μL.The mass spectrometry was performed using an ESI source,and the data were collected by switching the positive and negative ions modes,respectively.The data were collected by switching the scanning mode of positive and negative ions,and the components were identified according to the precise molecular weight,secondary mass spectrometry fragmentation ion information,combined with the literature reports,self-constructed database,and cleavage pattern.Results A total of 42 constituents were identified in Chaenomeles sinensis,including 12 organic acids and their derivatives,23 flavonoids and coumarins,and seven terpenoids and others.Meanwhile,36 constituents were identified in Chaenomeles speciosa,including 14 organic acids and their derivatives,14 flavonoids and coumarins,and eight terpenoids and others;Among these,both have 25 indentical constituents.Conclusion There were more chemical components in Chaenomeles sinensis than in Chaenomeles speciosa,and the same components accounted for 69.44％of the components in Chaenomeles speciosa,suggesting that Chaenomeles sinensis has significant medicinal value,with more tremendous potential for development and utilization.