Preparation of AgNPs-PCL-Gel fiber membrane and preliminary exploration of its anti-proliferative effect on glioma

SHI Mengrou; LI Xijing; CHENG Lin; CHENG Peng; ZHAO Xiumei; ZONG Wenhui

doi:10.13506/j.cnki.jpr.2025.03.004

Abstract：Objective To screen the optimal conditions for preparing AgNPs-PCL-Gel nanofiber membrane and ex-plore its inhibitory effect on the proliferation of glioma.Methods Cur was loaded onto PCL and Gel co spun nanofiber membranes using electrospinning technology,and then the Cur-PCL-Gel nanofiber membrane was placed in an aqueous so-lution of silver nitrate(AgNO3)to prepare AgNPs-PCL-Gel nanofiber membranes.Orthogonal optimization of electrospinning conditions was carried out,and the morphology of fibers and AgNPs was observed by scanning electron microscopy.The con-tent of AgNPs was analyzed by energy dispersive spectroscopy,and the swelling performance,hydrophilicity,degradation per-formance,in vitro release performance,and in vitro anti glioma U-251MG cell proliferation effect of the material were evalu-ated.Results AgNPs-PCL-Gel nanofibers were prepared under the optimal conditions of PCL∶Gel=5∶5,load voltage of 16 kV,liquid pushing speed of 0.000 8 mm·s-1,and receiving distance of 15 cm.The synthesized AgNPs exist on the nano-fibers in a spherical shape with a particle size of less than 30 nm.After 96 hours of reaction,the generated AgNPs reached their peak.The nanofiber membrane has good swelling and hydrophilic properties,and its degradation rate in artificial cere-brospinal fluid at pH 6.2 is faster than that at pH 7.4.In vitro release studies also show that it releases drugs faster and more completely in the release medium at pH 6.2.AgNPs-PCL-Gel nanofiber membrane has a significant cytotoxic effect on U-251 MG cells,with an IC50 value of 0.15 mg·mL-1.Conclusion AgNPs are evenly distributed on the nanofibers,and no ag-glomeration phenomenon was observed.Regulating the reaction time can control the generation of AgNPs.The higher the con-tent of AgNPs in the fiber,the more hydrophilic the fiber membrane becomes.The nanofiber membrane has good drug release performance and a sustained release effect.AgNPs-PCL-Gel nanofiber membrane has a good inhibitory effect on the prolif-eration of U-251MG cells and shows potential application in the field of anti glioma.