Enhanced immunogenicity of porcine reproductive and respiratory syndrome vaccine by chitosan oligosaccharides
SUN Zhaolin
WEI Yuhua
WANG Zhuo
DU Yuguang
DUAN Xiuying
JIA Peiyuan
ZHANG Guiqiang
Abstract：Objective To investigate the adjuvant effect of chitosan oligosaccharides(COS)on the inactivated porcine reproductive and respiratory syndrome vaccine(PRRSV).Methods BALB/c mice were immunized with PRRSV alone or in combination with COSs that possess varying degrees of acetylation(DA)and degrees of polymerization(DP).The adju-vant effect was evaluated by determining the serum total IgG levels and the titers of its various subtypes against PRRSV u-sing ELISA.Additionally,the immunological mechanisms were explored both in vitro and in vivo.Results Compared to the PRRSV alone,COSs significantly increased the antibody levels of total IgG and its subtypes,promoted lymphocyte prolifera-tion,increased the numbers of antigen-specific CD4+and CD8+T cells in the spleens,and initiated a two-way regulation of cytokine secretion.The molecular mechanism may be associated with the overexpression of Toll-like receptors(TLRs)on macrophages,which subsequently promotes PRRSV presentation.Conclusion COSs may serve as safe and effective candi-dates for the development of new adjuvants for PRRSV.
Keywords：Chitosan oligosaccharidesPorcine reproductive and respiratory syndrome vaccineAdjuvantDegree of acetylationDegree of polymerization
Publication Date：2025-03-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 217-223,256 )