Study on quality analysis method of Imperatae Rhizoma based on"quality-quantity"double standard
LIU Yuhan
WANG Shuai
BAO Yongrui
LI Tianjiao
GUAN Xin
MENG Xiansheng
Abstract：Objective To establish a"quality-quantity"double standard control method for Imperatae Rhizoma using the reference medicinal materials as the qualitative control materials and without relying on multiple reference materials for quantification.Methods Using high performance liquid chromatography(HPLC)technology,with the control medicinal ma-terials as the reference material,the characteristic spectrum of Imperatae Rhizoma was established,and the common peak chemical composition was identified by mass spectrometry.The authenticity of the medicinal materials was identified by eval-uating the similarity of characteristic spectrum between the control medicinal materials and the test medicinal materials,that is,"quality".With chlorogenic acid as the reference material,the relative quantitative study of chemical composition of char-acteristic peak was established to distinguish the advantages and inferior of medicinal materials,that is,"quantity".Results The established qualitative and quantitative analysis method met the requirements of methodological investigation.The similarity between the tested medicinal materials and the control medicinal materials was greater than 0.900,and the relative content limit of the chemical components of the characteristic peak of Imperatae Rhizoma was stipulated.Conclusion This method was accurate,fast and simple,and can make up for the deficiency of the current quality control of the medicinal ma-terials of Imperatae Rhizoma,and achieve the purpose of comprehensive control of the quality of Imperatae Rhizoma.
Keywords：Imperatae RhizomaCompared with medicinal materialsQuality-quantity double standardQuality controlFeature mapChlorogenic acid
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 36-41 )